Abstract
We demonstrate that IL-2-activated NK cells or lymphokine-activated killer cells recognize and kill syngeneic CD4(+) and CD8(+) T cells that have been activated by APCs. Induction with APC required TCR-specific Ag, and lysis was perforin mediated. Brefeldin A, which disrupts protein transport, inhibited the sensitivity induced by activation. In BALB/c, expression of NKG2D ligands correlated with lysis and could be inhibited by brefeldin A. As well, addition of anti-NKG2D mAb to a killing assay completely abrogated lysis. Transduction of mouse NKG2D into a human NK cell line, YTSeco, conferred upon it the ability to kill activated BALB/c T cells, indicating that NKG2D is necessary for recognition. Our data provide a basis for studying a role for NK cells in T cell regulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cytotoxicity Tests, Immunologic
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Cytotoxicity, Immunologic* / genetics
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Humans
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Interphase / immunology*
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Killer Cells, Lymphokine-Activated / immunology
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Killer Cells, Lymphokine-Activated / metabolism
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism
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Ligands
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Lymphocyte Activation* / genetics
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, SCID
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Mice, Transgenic
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Minor Histocompatibility Antigens / metabolism
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NK Cell Lectin-Like Receptor Subfamily K
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism
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Receptors, Immunologic / physiology
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Receptors, Natural Killer Cell
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / physiology
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Species Specificity
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism
Substances
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KLRK1 protein, human
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Klrk1 protein, mouse
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Ligands
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Minor Histocompatibility Antigens
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NK Cell Lectin-Like Receptor Subfamily K
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Receptors, Immunologic
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Receptors, Natural Killer Cell
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Recombinant Fusion Proteins
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minor H antigen H60