HLA-A3 supermotif defined by quantitative structure-activity relationship analysis

Protein Eng. 2003 Jan;16(1):11-8. doi: 10.1093/proeng/gzg005.

Abstract

Activation of a cytotoxic T cell requires specific binding of antigenic peptides to major histocompatibility complex (MHC) molecules. This paper reports a study of peptides binding to members of the HLA-A3 superfamily using a recently developed 2D-QSAR method, called the additive method. Four alleles with high phenotype frequency were included in the study: A*0301, A*1101, A*3101 and A*6801. The influence of each of the 20 amino acids at each position of the peptide on binding was studied. A refined A3 supertype motif was defined in the study.

MeSH terms

  • Alleles
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Amino Acids / chemistry
  • Amino Acids / genetics
  • Amino Acids / metabolism
  • Binding Sites
  • HLA-A3 Antigen / chemistry*
  • HLA-A3 Antigen / genetics
  • HLA-A3 Antigen / immunology
  • HLA-A3 Antigen / metabolism*
  • Humans
  • Models, Biological
  • Molecular Sequence Data
  • Oligopeptides / chemistry
  • Oligopeptides / genetics
  • Oligopeptides / metabolism
  • Phenotype
  • Protein Binding
  • Quantitative Structure-Activity Relationship
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism

Substances

  • Amino Acids
  • HLA-A3 Antigen
  • Oligopeptides
  • Recombinant Proteins