Abstract
Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCF(GID2)-proteasome pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Amino Acid Sequence
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Codon, Terminator
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Enzyme Induction
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Genes, Plant
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Gibberellins / metabolism*
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Gibberellins / pharmacology
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Ligases / metabolism
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Molecular Sequence Data
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Mutation
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Open Reading Frames
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Oryza / genetics
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Oryza / growth & development
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Oryza / metabolism*
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Peptide Hydrolases / metabolism
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Phenotype
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Phosphorylation
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism*
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Proteasome Endopeptidase Complex*
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Protein Structure, Tertiary
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Seeds / metabolism
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Signal Transduction*
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Ubiquitin / metabolism
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Ubiquitin-Protein Ligases
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alpha-Amylases / biosynthesis
Substances
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Codon, Terminator
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Gibberellins
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Plant Proteins
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Ubiquitin
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gibberellic acid
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Ubiquitin-Protein Ligases
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alpha-Amylases
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Peptide Hydrolases
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Proteasome Endopeptidase Complex
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ATP dependent 26S protease
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Ligases