The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromoviruses

N Sasaki; M Arimoto; H Nagano; M Mori; M Kaido; K Mise; T Okuno

doi:10.1007/s00705-002-0952-x

The movement protein gene is involved in the virus-specific requirement of the coat protein in cell-to-cell movement of bromoviruses

Arch Virol. 2003 Apr;148(4):803-12. doi: 10.1007/s00705-002-0952-x.

Authors

N Sasaki¹, M Arimoto, H Nagano, M Mori, M Kaido, K Mise, T Okuno

Affiliation

¹ Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

PMID: 12664302
DOI: 10.1007/s00705-002-0952-x

Abstract

Brome mosaic virus (BMV) requires the coat protein (CP) for cell-to-cell movement whereas Cowpea chlorotic mottle virus (CCMV), from the same genus, does not. Chimeric viruses created by exchanging the movement protein (MP) gene between the viruses can move from cell to cell. We show that interference in CP expression impaired the movement of the chimeric CCMV with the BMV MP gene but not of the chimeric BMV with the CCMV MP gene. We thus conclude that the MP gene plays a crucial role in determination of the virus-specific CP requirement in bromovirus cell-to-cell movement.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bromoviridae / genetics
Bromoviridae / pathogenicity
Bromoviridae / physiology*
Capsid Proteins / analysis
Capsid Proteins / genetics
Capsid Proteins / physiology*
Chenopodium quinoa / virology*
Genes, Viral
Green Fluorescent Proteins
Luminescent Proteins
Molecular Sequence Data
Plant Diseases / virology
Plant Viral Movement Proteins
Protoplasts / virology
RNA, Viral / analysis
RNA, Viral / genetics*
Reassortant Viruses
Viral Proteins / analysis
Viral Proteins / genetics*

Substances

Capsid Proteins
Luminescent Proteins
Plant Viral Movement Proteins
RNA, Viral
Viral Proteins
Green Fluorescent Proteins