Basiliximab interferes with the detection of soluble IL-2 receptor by the Immulite Immunoassay system

J Immunol Methods. 2003 Apr 1;275(1-2):133-6. doi: 10.1016/s0022-1759(03)00016-4.

Abstract

The use of the Immulite Automated Immunoassay system is becoming an increasingly popular method for accurate and fast measurement of sIL-2R in serum. Here, we report that detection of sIL-2R using the Immulite Immunoassay system in serum samples of patients who are treated with basiliximab, a monoclonal antibody against the alpha-chain of the IL-2 receptor, may lead to false measurements.

MeSH terms

  • Antibodies, Monoclonal / blood*
  • Antibodies, Monoclonal / therapeutic use
  • Basiliximab
  • Binding, Competitive
  • Humans
  • Immunoassay / methods*
  • Immunosuppressive Agents / blood
  • Immunosuppressive Agents / therapeutic use
  • Liver Transplantation / immunology
  • Receptors, Interleukin-2 / blood*
  • Recombinant Fusion Proteins*
  • Solubility
  • T-Lymphocytes / immunology
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • Immunosuppressive Agents
  • Receptors, Interleukin-2
  • Recombinant Fusion Proteins
  • Basiliximab