Detection of molecular variability in rice tungro bacilliform viruses from India using polymerase chain reaction-restriction fragment length polymorphism

Rekha Joshi; Vishnu Kumar; Indranil Dasgupta

doi:10.1016/s0166-0934(02)00290-2

Detection of molecular variability in rice tungro bacilliform viruses from India using polymerase chain reaction-restriction fragment length polymorphism

J Virol Methods. 2003 Apr;109(1):89-93. doi: 10.1016/s0166-0934(02)00290-2.

Authors

Rekha Joshi¹, Vishnu Kumar, Indranil Dasgupta

Affiliation

¹ Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, India.

PMID: 12668273
DOI: 10.1016/s0166-0934(02)00290-2

Abstract

Rice tungro bacilliform virus (RTBV) with rice tungro spherical virus (RTSV) causes the destructive tungro disease of rice. In order to ascertain the molecular variability of RTBV in India, primers were designed to amplify a polymorphic DNA fragment of the virus. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis on a number of field isolates indicated mixed infections and molecular heterogeneity in the viral genome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Badnavirus / genetics
Badnavirus / isolation & purification*
Genetic Variation*
India
Molecular Sequence Data
Oryza / virology*
Plant Diseases / virology
Plant Leaves / virology
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length*
Sequence Analysis, DNA
Waikavirus / genetics
Waikavirus / isolation & purification*

Associated data

GENBANK/AJ292232
GENBANK/AJ314596