Undifferentiated (embryonal) sarcoma of the liver in middle-aged adults: smooth muscle differentiation determined by immunohistochemistry and electron microscopy

Hum Pathol. 2003 Mar;34(3):246-52. doi: 10.1053/hupa.2003.44.

Abstract

Undifferentiated (embryonal) sarcoma of the liver (UESL) is a rare pediatric liver malignancy that is extremely uncommon in middle-aged individuals. We studied 2 cases of UESL in middle-aged adults (1 case in a 49-year-old woman and the other in a 62-year-old man) by histology, immunohistochemistry, and electron microscopy to clarify the cellular characteristics of this peculiar tumor. One tumor showed a mixture of spindle cells, polygonal cells, and multinucleated giant cells within a myxoid matrix and also revealed focal areas of a storiform pattern in a metastatic lesion. The other tumor was composed mainly of anaplastic large cells admixed with few fibrous or spindle-shaped components and many multinucleated giant cells. In both cases, some tumor cells contained eosinophilic hyaline globules that were diastase resistant and periodic acid-Schiff positive. Immunohistochemically, the tumor cells showed positive staining for smooth muscle markers, such as desmin, alpha-smooth muscle actin, and muscle-specific actin, and also for histiocytic markers, such as alpha-1-antitrypsin, alpha-1-antichymotrypsin, and CD68. Electron microscope examination revealed thin myofilaments with focal densities and intermediate filaments in the cytoplasm of tumor cells. Our studies suggest that UESL exhibits at least a partial smooth muscle phenotype in middle-aged adults, and this specific differentiation may be more common in this age group than in children. Tumor cells of UESL with smooth muscle differentiation in middle-aged adults show phenotypic diversity comparable to those of malignant fibrous histiocytoma with myofibroblastic differentiation.

Publication types

  • Case Reports

MeSH terms

  • Actins / analysis
  • Antigens, CD / analysis
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Cell Differentiation*
  • Cell Nucleus / ultrastructure
  • Cytoplasm / ultrastructure
  • Desmin / analysis
  • Endoplasmic Reticulum, Rough / ultrastructure
  • Female
  • Golgi Apparatus / ultrastructure
  • Humans
  • Immunohistochemistry*
  • Liver Neoplasms / pathology*
  • Male
  • Microscopy, Electron*
  • Middle Aged
  • Muscle, Smooth / pathology*
  • Neoplasms, Germ Cell and Embryonal / pathology*
  • Periodic Acid-Schiff Reaction
  • alpha 1-Antichymotrypsin / analysis
  • alpha 1-Antitrypsin / analysis

Substances

  • Actins
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD68 antigen, human
  • Desmin
  • alpha 1-Antichymotrypsin
  • alpha 1-Antitrypsin