Enterobacterial repetitive intergenic consensus 1R PCR assay for detection of Raoultella sp. isolates among strains identified as Klebsiella oxytoca in the clinical laboratory

J Clin Microbiol. 2003 Apr;41(4):1740-2. doi: 10.1128/JCM.41.4.1740-1742.2003.

Abstract

The enterobacterial repetitive intergenic consensus 1R PCR method, which provided recognizable profiles for reference strains of the three species of Raoultella and the two genetic groups of Klebsiella oxytoca, was applied to 19 clinical isolates identified as K. oxytoca. By this method, as confirmed by species-specific gene sequencing, two Raoultella ornithinolytica and two unclassifiable K. oxytoca isolates were identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • DNA, Intergenic / analysis*
  • Enterobacteriaceae / classification*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae Infections / microbiology*
  • Humans
  • Klebsiella Infections / microbiology
  • Klebsiella oxytoca / classification*
  • Klebsiella oxytoca / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Repetitive Sequences, Nucleic Acid / genetics*
  • Species Specificity

Substances

  • DNA, Intergenic

Associated data

  • GENBANK/AF491278
  • GENBANK/AY077481
  • GENBANK/AY207376
  • GENBANK/AY208272
  • GENBANK/AY208273
  • GENBANK/AY208274
  • GENBANK/AY208275
  • GENBANK/AY208276
  • GENBANK/AY210425
  • GENBANK/AY210426
  • GENBANK/AY210427
  • GENBANK/AY210428
  • GENBANK/AY210429
  • GENBANK/AY210430
  • GENBANK/AY210431
  • GENBANK/AY210432
  • GENBANK/AY210433