Abstract
Gaf-1/Rip11 encoded by the clone KIAA0857 participates in endosomal recycling through the interaction with both gamma-SNAP, a member of the soluble NSF attachment protein family, and a small GTPase, Rab11. Gaf-1/Rip11 and other Rab11-interacting proteins constitute a novel protein family that is involved in the endocytic pathways. Here we report the presence of an alternative splice variant of Gaf-1/Rip11 named Gaf-1b. Gaf-1b also interacts with gamma-SNAP and is expressed ubiquitously in tissues except for liver. Subcellular fractionation analysis revealed that Gaf-1b, as well as Gaf-1/Rip11, is mainly present in the microsomal fraction. Overexpression of Gaf-1b, like that of Gaf-1/Rip11, affected the morphology of recycling endosomes. These results suggest that Gaf-1b has a similar function to Gaf-1/Rip11.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Alternative Splicing*
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Carrier Proteins / analysis
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Carrier Proteins / physiology*
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Cell Line
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Endosomes / ultrastructure
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HeLa Cells
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Humans
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Membrane Proteins / analysis
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Membrane Proteins / physiology*
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Mitochondrial Proteins*
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Trans-Activators / analysis
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Trans-Activators / genetics*
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Vesicular Transport Proteins*
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Membrane Proteins
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Mitochondrial Proteins
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RAB11FIP5 protein, human
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Trans-Activators
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Vesicular Transport Proteins