Expression and function of prostate-apoptosis-response-gene-4 in lymphatic cells

Leuk Lymphoma. 2002 Sep;43(9):1737-41. doi: 10.1080/1042819021000006510.

Abstract

Inhibition of apoptosis contributes to the pathogenesis of lymphatic malignancies. In particular, the elevated expression of Bcl-2 is considered to be a marker of poor prognosis, since increased levels of Bcl-2 confer longevity as well as chemoresistance. After demonstrating an inverse expressional pattern of Bcl-2 and prostate-apoptosis-response-4 (Par-4) in ex vivo cells of patients suffering from acute lymphatic leukemia (ALL) as well as a deregulated expression of Par-4 in acute and chronic lymphatic neoplasias, the molecular mechanisms underlying these results were investigated. Thus, it was demonstrated that in neoplastic lymphatic cells Par-4 exerts a proapoptotic role augmenting chemosensitivity by down-regulating Bcl-2, promoting disruption of mitochondrial membrane potential and enforcing caspase-activation. Moreover, Par-4 enables cells to circumvent inhibition of the central executioner caspase-3 by alternative activation of caspases following a decrease in expression levels of inhibitors of apoptosis proteins (IAP).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Apoptosis Regulatory Proteins
  • Carrier Proteins / biosynthesis*
  • Carrier Proteins / physiology*
  • Down-Regulation
  • Enzyme Activation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Jurkat Cells
  • Leukemia, Lymphocytic, Chronic, B-Cell / metabolism*
  • Models, Biological
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • RNA, Messenger / metabolism
  • T-Lymphocytes / metabolism

Substances

  • Apoptosis Regulatory Proteins
  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • prostate apoptosis response-4 protein