Band 3 is a host receptor binding merozoite surface protein 1 during the Plasmodium falciparum invasion of erythrocytes

Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5164-9. doi: 10.1073/pnas.0834959100. Epub 2003 Apr 11.

Abstract

We report the molecular identification of a sialic acid-independent host-parasite interaction in the Plasmodium falciparum malaria parasite invasion of RBCs. Two nonglycosylated exofacial regions of human band 3 in the RBC membrane were identified as a crucial host receptor binding the C-terminal processing products of merozoite surface protein 1 (MSP1). Peptides derived from the receptor region of band 3 inhibited the invasion of RBCs by P. falciparum. A major segment of the band 3 receptor (5ABC) bound to native MSP1(42) and blocked the interaction of native MSP1(42) with intact RBCs in vitro. Recombinant MSP1(19) (the C-terminal domain of MSP1(42)) bound to 5ABC as well as RBCs. The binding of both native MSP1(42) and recombinant MSP1(19) was not affected by the neuraminidase treatment of RBCs, but sensitive to chymotrypsin treatment. In addition, recombinant MSP1(38) showed similar interactions with the band 3 receptor and RBCs, although the interaction was relatively weak. These findings suggest that the chymotrypsin-sensitive MSP1-band 3 interaction plays a role in a sialic acid-independent invasion pathway and reveal the function of MSP1 in the Plasmodium invasion of RBCs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anion Exchange Protein 1, Erythrocyte / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Erythrocytes / parasitology*
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Merozoite Surface Protein 1 / metabolism*
  • Molecular Sequence Data
  • N-Acetylneuraminic Acid / metabolism
  • Plasmodium falciparum / physiology*
  • Protein Binding
  • Recombinant Proteins / metabolism

Substances

  • Anion Exchange Protein 1, Erythrocyte
  • Merozoite Surface Protein 1
  • Recombinant Proteins
  • N-Acetylneuraminic Acid

Associated data

  • GENBANK/AF286876
  • GENBANK/AF325919