Isolation of a new hemimethylated DNA binding protein which regulates dnaA gene expression

Emmanuelle d'Alençon; Aziz Taghbalout; Claire Bristow; Renée Kern; Revital Aflalo; Masamichi Kohiyama

doi:10.1128/JB.185.9.2967-2971.2003

Isolation of a new hemimethylated DNA binding protein which regulates dnaA gene expression

J Bacteriol. 2003 May;185(9):2967-71. doi: 10.1128/JB.185.9.2967-2971.2003.

Authors

Emmanuelle d'Alençon¹, Aziz Taghbalout, Claire Bristow, Renée Kern, Revital Aflalo, Masamichi Kohiyama

Affiliation

¹ Institut J. Monod, Université Paris VI-VII, 75251 Paris Cedex 05, France.

Abstract

In this report, we show that yccV, a gene of unknown function, encodes a protein having an affinity for a hemimethylated oriC DNA and that the protein negatively controls dnaA gene expression in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacterial Proteins / biosynthesis
Bacterial Proteins / genetics*
Base Sequence
DNA Methylation
DNA Replication
DNA Transposable Elements
DNA-Binding Proteins / biosynthesis
DNA-Binding Proteins / genetics*
Down-Regulation
Escherichia coli / chemistry
Escherichia coli / genetics*
Gene Expression Regulation
Genes, Bacterial*
Molecular Sequence Data

Substances

Bacterial Proteins
DNA Transposable Elements
DNA-Binding Proteins
DnaA protein, Bacteria