First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

T P Nicholson; C Winfield; J Westcott; J Crosby; T J Simpson; R J Cox

doi:10.1039/b300847a

First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

Chem Commun (Camb). 2003 Mar 21:(6):686-7. doi: 10.1039/b300847a.

Authors

T P Nicholson¹, C Winfield, J Westcott, J Crosby, T J Simpson, R J Cox

Affiliation

¹ School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS.

PMID: 12703773
DOI: 10.1039/b300847a

Abstract

The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acyl Carrier Protein / metabolism
Acyltransferases / chemistry
Acyltransferases / genetics
Acyltransferases / metabolism*
Anthraquinones / chemistry
Anthraquinones / metabolism*
Bacterial Proteins*
Malonyl Coenzyme A / metabolism
Polyketide Synthases
Spectrometry, Mass, Electrospray Ionization
Streptomyces / enzymology

Substances

Acyl Carrier Protein
Anthraquinones
Bacterial Proteins
Malonyl Coenzyme A
Polyketide Synthases
Acyltransferases
polyketide beta-ketoacylsynthase
actinorhodin