Binding of intimin from enteropathogenic Escherichia coli to lymphocytes and its functional consequences

Nathalie S Gonçalves; Christine Hale; Gordon Dougan; Gad Frankel; Thomas T MacDonald

doi:10.1128/IAI.71.5.2960-2965.2003

Binding of intimin from enteropathogenic Escherichia coli to lymphocytes and its functional consequences

Infect Immun. 2003 May;71(5):2960-5. doi: 10.1128/IAI.71.5.2960-2965.2003.

Authors

Nathalie S Gonçalves¹, Christine Hale, Gordon Dougan, Gad Frankel, Thomas T MacDonald

Affiliation

¹ School of Medicine, University of Southampton, Southampton General Hospital, Southampton SO16 6YD, United Kingdom.

Abstract

Intimin-conjugated fluorescent beads bind to spleen CD4 T cells and Peyer's patch, mesenteric lymph node, and cecal follicle lymphocytes, with less binding to lamina propria T cells and intraepithelial lymphocytes. Intimin costimulates proliferation of spleen CD4 T cells and cells from organized lymphoid tissues but does not costimulate cells from the lamina propria of normal or inflamed colon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adhesins, Bacterial / metabolism*
Adhesins, Bacterial / pharmacology
Animals
CD3 Complex / immunology
CD4-Positive T-Lymphocytes / metabolism*
Carrier Proteins / metabolism*
Carrier Proteins / pharmacology
Escherichia coli Proteins / metabolism*
Female
Lymphocyte Activation / drug effects
Mice
Mice, Inbred BALB C

Substances

Adhesins, Bacterial
CD3 Complex
Carrier Proteins
Escherichia coli Proteins
eaeA protein, E coli