Vitamin D(3) up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species

Hyunkeun Song; Daeho Cho; Jun Ho Jeon; Seung Hyun Han; Dae Young Hur; Young Sang Kim; Inpyo Choi

doi:10.1016/s0165-2478(03)00024-5

Vitamin D(3) up-regulating protein 1 (VDUP1) antisense DNA regulates tumorigenicity and melanogenesis of murine melanoma cells via regulating the expression of fas ligand and reactive oxygen species

Immunol Lett. 2003 May 1;86(3):235-47. doi: 10.1016/s0165-2478(03)00024-5.

Authors

Hyunkeun Song¹, Daeho Cho, Jun Ho Jeon, Seung Hyun Han, Dae Young Hur, Young Sang Kim, Inpyo Choi

Affiliation

¹ Laboratory of Immunology, Korea Research Institute of Bioscience and Biotechnology, Eoun-Dong 52, Yusong, Taejon 305-333, South Korea.

PMID: 12706526
DOI: 10.1016/s0165-2478(03)00024-5

Abstract

The expression of vitamin D(3) up-regulating protein-1 (VDUP1) was up-regulated by 1alpha,25-dihydroxyvitamin D(3) (VD3) treatment in B16 mouse melanoma cells. The functional effect of VDUP1 on B16F10 melanoma cells was demonstrated by reduction of Fas ligand and CD44 expression in cells transfected with VDUP1 antisense cDNA. Furthermore, intracellular reactive oxygen species level and cell proliferation were decreased in antisense transfectants compared with those in vector controls. However, melanin synthesis was up-regulated in antisense transfectants. In addition, VDUP1 antisense transfectants showed an increased susceptibility to natural killer (NK) cells in vitro. When VDUP1 antisense transfectants were implanted into syngeneic mice, significant reduction of tumor cell growth was observed with the infiltrate of T cells and NK cells in tumor area. Taken together, these results demonstrate that VDUP1 has critical physiological roles and can be a novel therapeutic target for melanoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carrier Proteins / drug effects
Carrier Proteins / genetics
Carrier Proteins / immunology
Carrier Proteins / metabolism*
Cell Line, Tumor
DNA, Antisense
Fas Ligand Protein
Flow Cytometry
Gene Expression Regulation, Neoplastic
Hyaluronan Receptors / biosynthesis
Immunoblotting
Immunohistochemistry
Killer Cells, Natural / immunology
Male
Melanins / biosynthesis
Melanoma / immunology
Melanoma / metabolism*
Membrane Glycoproteins / metabolism*
Mice
Polyamines
Reactive Oxygen Species / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Thioredoxins*
Transfection
Vitamin D / analogs & derivatives*
Vitamin D / pharmacology

Substances

2,10-dioximino-3,9-dimethyl-4,8-diazaundeca-3,8-diene
Carrier Proteins
DNA, Antisense
FASLG protein, human
Fas Ligand Protein
Fasl protein, mouse
Hyaluronan Receptors
Melanins
Membrane Glycoproteins
Polyamines
Reactive Oxygen Species
TXNIP protein, human
dihydroxy-vitamin D3
Vitamin D
Thioredoxins