Abstract
We sequenced the 29,751-base genome of the severe acute respiratory syndrome (SARS)-associated coronavirus known as the Tor2 isolate. The genome sequence reveals that this coronavirus is only moderately related to other known coronaviruses, including two human coronaviruses, HCoV-OC43 and HCoV-229E. Phylogenetic analysis of the predicted viral proteins indicates that the virus does not closely resemble any of the three previously known groups of coronaviruses. The genome sequence will aid in the diagnosis of SARS virus infection in humans and potential animal hosts (using polymerase chain reaction and immunological tests), in the development of antivirals (including neutralizing antibodies), and in the identification of putative epitopes for vaccine development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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5' Untranslated Regions
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Animals
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Base Sequence
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Conserved Sequence
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Coronavirus / classification
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Coronavirus / genetics
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Coronavirus M Proteins
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Coronavirus Nucleocapsid Proteins
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DNA, Complementary
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Frameshifting, Ribosomal
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Genome, Viral*
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Humans
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics
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Nucleocapsid Proteins / chemistry
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Nucleocapsid Proteins / genetics
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Open Reading Frames
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Phylogeny
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RNA, Viral / genetics*
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RNA, Viral / isolation & purification
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RNA-Dependent RNA Polymerase / chemistry
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RNA-Dependent RNA Polymerase / genetics
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Regulatory Sequences, Nucleic Acid
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Sequence Analysis, DNA
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Severe Acute Respiratory Syndrome / virology
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Severe acute respiratory syndrome-related coronavirus / classification
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Severe acute respiratory syndrome-related coronavirus / genetics*
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Severe acute respiratory syndrome-related coronavirus / isolation & purification
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Spike Glycoprotein, Coronavirus
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Viral Envelope Proteins / chemistry
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Viral Envelope Proteins / genetics
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Viral Matrix Proteins / chemistry
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Viral Matrix Proteins / genetics
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Viral Proteins / chemistry
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Viral Proteins / genetics*
Substances
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3' Untranslated Regions
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5' Untranslated Regions
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Coronavirus M Proteins
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Coronavirus Nucleocapsid Proteins
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DNA, Complementary
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M protein, Human coronavirus OC43
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M protein, SARS-CoV
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Membrane Glycoproteins
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N protein, SARS-CoV
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Nucleocapsid Proteins
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RNA, Viral
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Spike Glycoprotein, Coronavirus
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Viral Envelope Proteins
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Viral Matrix Proteins
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Viral Proteins
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RNA-Dependent RNA Polymerase