Abstract
We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Escherichia coli / genetics*
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Escherichia coli / isolation & purification
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Escherichia coli / pathogenicity
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Escherichia coli Proteins / genetics
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Genes, Bacterial*
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Humans
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Plasmids / genetics*
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Sensitivity and Specificity
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Trans-Activators / genetics
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Virulence / genetics
Substances
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AggR protein, E coli
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DNA Primers
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DNA, Bacterial
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Escherichia coli Proteins
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Trans-Activators