Abstract
There has been controversy over the possible lymphoid origin of certain dendritic cell (DC) subtypes. To resolve this issue, DC and plasmacytoid pre-DC isolated from normal mouse tissues were analyzed for transient (mRNA) and permanent (DNA rearrangement) markers of early stages of lymphoid development. About 27% of the DNA of CD8(+) DC from thymus, and 22-35% of the DNA of plasmacytoid pre-DC from spleen and thymus, was found to contain IgH gene D-J rearrangements, compared with 40% for T cells. However, the DC DNA did not contain IgH gene V-D-J rearrangements nor T cell Ag receptor beta gene D-J rearrangements. The same DC lineage populations containing IgH D-J rearrangements expressed mRNA for CD3 chains, and for pre-T alpha. In contrast, little of the DNA of the conventional DC derived from spleen, lymph nodes, or skin, whether CD8(+) or CD8(-), contained IgH D-J rearrangements and splenic conventional DC expressed very little CD3 epsilon or pre-T alpha mRNA. Therefore, many plasmacytoid pre-DC and thymic CD8(+) DC have shared early steps of development with the lymphoid lineages, and differ in origin from conventional peripheral DC.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow Cells / immunology
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Bone Marrow Cells / metabolism
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Bone Marrow Transplantation
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CD3 Complex*
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell Lineage / genetics
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Cell Lineage / immunology
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Cells, Cultured
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Complementarity Determining Regions / biosynthesis
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Complementarity Determining Regions / genetics
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Dendritic Cells / cytology*
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Female
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Gene Rearrangement, B-Lymphocyte, Heavy Chain
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Genes, T-Cell Receptor alpha
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Lymphoid Tissue / cytology*
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Lymphoid Tissue / immunology
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Lymphoid Tissue / metabolism
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Male
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Membrane Glycoproteins / biosynthesis
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Membrane Glycoproteins / genetics
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Phagocytosis / genetics
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Phagocytosis / immunology
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Plasma Cells / cytology*
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Plasma Cells / immunology
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Plasma Cells / metabolism
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RNA, Messenger / biosynthesis
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Radiation Chimera / immunology
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Receptors, Antigen, T-Cell / biosynthesis
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell, alpha-beta
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Stem Cells / cytology
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Stem Cells / immunology
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Stem Cells / metabolism
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Thymus Gland / cytology*
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Thymus Gland / immunology
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Thymus Gland / metabolism
Substances
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CD3 Complex
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CD3E protein, human
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Complementarity Determining Regions
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Membrane Glycoproteins
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RNA, Messenger
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Receptors, Antigen, T-Cell
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Receptors, Antigen, T-Cell, alpha-beta
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pre-T cell receptor alpha