Photochemically induced double lateral wall lesions in the guinea pig cochlea

Acta Otolaryngol. 2003 Apr;123(3):355-61. doi: 10.1080/0001648021000028131.

Abstract

Objective: Multiple patches of atrophy have been reported in the stria vascularis (SV) in elderly persons with presbycusis The aim of this study was to investigate the correlation between sensorineural hearing loss and this strial condition.

Materials and methods: We established a new animal model comprising two small lesions in the SV in the second turn of the cochlea by means of photochemical reaction. Using this model, we investigated morphological and physiological changes in the cochlea at 3, 7 and 14 days after SV damage.

Results: Scanning electron microscopy studies revealed that the strial cells between the two damaged areas of the SV remained intact, although the outer hair cells (OHCs) facing the intact SV area were damaged. Furthermore, damage to the first and second rows of OHCs gradually progressed throughout the 14-day observation period. The endocochlear potential (EP) measured at a point midway between the 2 lesions at 3 and 7 days was found to be significantly lower compared with control values, but had returned to a normal level at 14 days

Conclusion: The reversible EP change and localized OHC loss seen in the present investigation may help to understand acute idiopathic or progressive sensorineural hearing loss.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cochlear Diseases / chemically induced
  • Cochlear Diseases / pathology*
  • Cochlear Diseases / physiopathology*
  • Cochlear Microphonic Potentials* / drug effects
  • Fluorescent Dyes / administration & dosage
  • Guinea Pigs
  • Hair Cells, Auditory, Inner / drug effects
  • Hair Cells, Auditory, Outer / drug effects
  • Male
  • Microscopy, Electron, Scanning
  • Models, Animal
  • Photochemistry
  • Rose Bengal / administration & dosage
  • Stria Vascularis / drug effects
  • Stria Vascularis / pathology*
  • Time Factors

Substances

  • Fluorescent Dyes
  • Rose Bengal