Crosstalk between neurokinin receptors is relevant to hematopoietic regulation: cloning and characterization of neurokinin-2 promoter

J Neuroimmunol. 2003 May;138(1-2):65-75. doi: 10.1016/s0165-5728(03)00096-1.

Abstract

Neurokinin (NK)-1 and NK-2 receptors regulate hematopoiesis by interacting with neurotransmitters that belong to the tachykinin. This report studies the relationship between NK-1 and NK-2 in primary human bone marrow (BM) stroma, which supports hematopoiesis. Use of NK receptor antagonists and deficient stromal cells indicate that the neurotransmitter, substance P (SP), could exert dual hematopoietic effects (inhibitory or stimulatory), depending on the interacting receptor and crosstalk between NK-1 and NK-2. Cloning and identification of the minimal promoter for NK-2 and comparison with NK-1 promoter showed that the hematopoietic functions of NK receptors involve receptor crosstalk and the particular cytokine (IL-3, GM-CSF, TGF-beta or IL-1alpha). Crosstalk between NK-1 and NK-2 adds to communication within neural-hematopoietic axis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 5' Untranslated Regions / drug effects
  • Base Sequence
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / metabolism
  • Cell Division / physiology
  • Cells, Cultured
  • Cloning, Molecular / methods
  • Cytokines / pharmacology
  • Down-Regulation / drug effects
  • Gene Deletion
  • Hematopoiesis / genetics
  • Hematopoiesis / physiology*
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Molecular Sequence Data
  • Neurokinin-1 Receptor Antagonists
  • Promoter Regions, Genetic* / drug effects
  • Promoter Regions, Genetic* / genetics
  • RNA, Messenger / metabolism
  • Receptor Cross-Talk / physiology*
  • Receptors, Neurokinin-1 / genetics
  • Receptors, Neurokinin-1 / physiology*
  • Receptors, Neurokinin-2 / antagonists & inhibitors
  • Receptors, Neurokinin-2 / chemistry*
  • Receptors, Neurokinin-2 / genetics
  • Receptors, Neurokinin-2 / physiology*
  • Stromal Cells / drug effects
  • Stromal Cells / metabolism
  • Substance P / pharmacology
  • Transfection
  • Up-Regulation / drug effects

Substances

  • 5' Untranslated Regions
  • Cytokines
  • Neurokinin-1 Receptor Antagonists
  • RNA, Messenger
  • Receptors, Neurokinin-1
  • Receptors, Neurokinin-2
  • Substance P

Associated data

  • GENBANK/AF361880