STAT3 ubiquitylation and degradation by mumps virus suppress cytokine and oncogene signaling

J Virol. 2003 Jun;77(11):6385-93. doi: 10.1128/jvi.77.11.6385-6393.2003.

Abstract

Mumps virus is a common infectious agent of humans, causing parotitis, meningitis, encephalitis, and orchitis. Like other paramyxoviruses in the genus Rubulavirus, mumps virus catalyzes the proteasomal degradation of cellular STAT1 protein, a means for escaping antiviral responses initiated by alpha/beta and gamma interferons. We demonstrate that mumps virus also eliminates cellular STAT3, a protein that mediates transcriptional responses to cytokines, growth factors, nonreceptor tyrosine kinases, and a variety of oncogenic stimuli. STAT1 and STAT3 are independently targeted by a single mumps virus protein, called V, that assembles STAT-directed ubiquitylation complexes from cellular components, including STAT1, STAT2, STAT3, DDB1, and Cullin4A. Consequently, mumps virus V protein prevents responses to interleukin-6 and v-Src signals and can induce apoptosis in STAT3-dependent multiple myeloma cells and transformed murine fibroblasts. These findings demonstrate a unique cytokine and oncogene evasion property of mumps virus that provides a molecular basis for its observed oncolytic properties.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Apoptosis
  • Cell Line, Transformed
  • Cytokines / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Genes, src / physiology*
  • Humans
  • Interferon-beta / metabolism
  • Interleukin-6 / metabolism
  • Mice
  • Mumps virus / pathogenicity*
  • Mumps virus / physiology
  • Oncogenes / physiology
  • STAT1 Transcription Factor
  • STAT3 Transcription Factor
  • Signal Transduction*
  • Trans-Activators / metabolism*
  • Tumor Cells, Cultured
  • Ubiquitin / metabolism*
  • Viral Proteins / metabolism

Substances

  • Cytokines
  • DNA-Binding Proteins
  • Interleukin-6
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • Stat1 protein, mouse
  • Stat3 protein, mouse
  • Trans-Activators
  • Ubiquitin
  • Viral Proteins
  • Interferon-beta