Molecular cloning and characterization of an SRCAP chromatin remodeling homologue in Toxoplasma gondii

Parasitol Res. 2003 May;90(1):1-8. doi: 10.1007/s00436-002-0814-1. Epub 2003 Jan 28.

Abstract

We have identified and mapped a gene in Toxoplasma gondii that encodes a homologue of SRCAP (Snf2-related CBP activator protein), a member of the SNF/SWI family of chromatin remodeling factors. The genomic locus (TgSRCAP) is present as a single copy and contains 16 introns. The predicted cDNA contains an open reading frame of 8,775 bp and encodes a protein of 2,924 amino acids. We have identified additional SRCAP-like sequences in Apicomplexa for comparison by screening genomic databases. An analysis of SRCAP homologues between species reveals signature features that may be indicative of SRCAP members. Expression of mRNA encoding TgSRCAP is upregulated when tachyzoite (invasive form) parasites are induced to differentiate into bradyzoites (encysted form) in vitro. Recombinant TgSRCAP protein is functionally equivalent to the human homologue, being capable of increasing transcription mediated by CREB.

MeSH terms

  • Adenosine Triphosphatases / analysis
  • Adenosine Triphosphatases / chemistry*
  • Adenosine Triphosphatases / genetics*
  • Amino Acid Sequence
  • Animals
  • CREB-Binding Protein
  • Chromatin / genetics*
  • Cloning, Molecular
  • Gene Expression Regulation
  • Genes, Protozoan*
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid
  • Toxoplasma / genetics*
  • Toxoplasma / isolation & purification
  • Trans-Activators / metabolism
  • Transcription, Genetic

Substances

  • Chromatin
  • Nuclear Proteins
  • RNA, Messenger
  • Trans-Activators
  • CREB-Binding Protein
  • CREBBP protein, human
  • Adenosine Triphosphatases
  • SRCAP protein, human

Associated data

  • GENBANK/AY061650