A novel method of generating prostate cancer metastases from orthotopic implants

Prostate. 2003 Jul 1;56(2):110-4. doi: 10.1002/pros.10235.

Abstract

Background: Spontaneous metastasis following implantation at the orthotopic site is a highly desired feature in prostate cancer (CaP) models, since it would enable studies of mechanisms associated with tumor cell dissemination.

Methods: LuCaP 23.8 and LuCaP 35, hormone-sensitive CaP xenografts that express PSA and the wild-type androgen receptor, were grown orthotopically in SCID mice. When tumor volumes reached approximately 250-500 mg, primary tumors were removed to allow micrometastases to grow.

Results: Using this procedure we generated macroscopic metastases (>20 mg) in 71% (LuCaP 23.8) and 100% (LuCaP 35) of animals, respectively.

Conclusions: These models may be used to evaluate new treatment modalities and study mechanisms associated with development of metastases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Disease Models, Animal
  • Humans
  • Liver Neoplasms / secondary
  • Lung Neoplasms / secondary
  • Male
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation / methods*
  • Pancreatic Neoplasms / secondary
  • Prostate-Specific Antigen / analysis
  • Prostatic Neoplasms / chemistry
  • Prostatic Neoplasms / secondary*
  • Transplantation, Heterologous

Substances

  • Prostate-Specific Antigen