Abstract
We identified and characterized two new ING family genes, p29ING4 and p28ING5,coding for two proteins of 249 and 240 amino acids, respectively. Both p29ING4 and p28ING5 proteins have a plant homeodomain finger motif also found in other ING proteins, and which is common in proteins involved in chromatin remodeling. p29ING4 or p28ING5 overexpression resulted in a diminished colony-forming efficiency, a decreased cell population in S phase, and the induction of apoptosis in a p53-dependent manner. Both p29ING4 and p28ING5 activate the p21/waf1 promoter, and induce p21/WAF1 expression. p29ING4 and p28ING5 enhance p53 acetylation at Lys-382 residues, and physically interact with p300, a member of histone acetyl transferase complexes, and p53 in vivo. These results indicate that p29ING4 and p28ING5 may be significant modulators of p53 function.
MeSH terms
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Acetylation
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Acetyltransferases / metabolism*
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Amino Acid Sequence
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Cell Cycle Proteins / metabolism*
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Cell Division / physiology
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Cloning, Molecular
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Colorectal Neoplasms / genetics
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Colorectal Neoplasms / metabolism
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DNA, Complementary / genetics
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Growth Inhibitors / genetics
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Growth Inhibitors / metabolism*
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Histone Acetyltransferases
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Humans
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Molecular Sequence Data
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Protein Binding
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Sequence Homology, Amino Acid
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Transcription Factors
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Transfection
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / metabolism*
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Tumor Suppressor Proteins / genetics
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Tumor Suppressor Proteins / metabolism*
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p300-CBP Transcription Factors
Substances
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Cell Cycle Proteins
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DNA, Complementary
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Growth Inhibitors
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Homeodomain Proteins
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ING4 protein, human
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ING5 protein, human
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Transcription Factors
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Tumor Suppressor Protein p53
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Tumor Suppressor Proteins
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Acetyltransferases
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Histone Acetyltransferases
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p300-CBP Transcription Factors
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p300-CBP-associated factor