Abstract
At the transition from meiosis to cleavage mitoses, Drosophila requires the cell cycle regulators encoded by the genes, giant nuclei (gnu), plutonium (plu) and pan gu (png). Embryos lacking Gnu protein undergo DNA replication and centrosome proliferation without chromosome condensation or mitotic segregation. We have identified the gnu gene encoding a novel phosphoprotein dephosphorylated by Protein phosphatase 1 at egg activation. Gnu is normally expressed in the nurse cells and oocyte of the ovary and is degraded during the embryonic cleavage mitoses. Ovarian death and sterility result from gnu gain of function. gnu function requires the activity of pan gu and plu.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Nucleus / metabolism*
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DNA Replication
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Drosophila Proteins / chemistry
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / cytology
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Drosophila melanogaster / embryology*
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Drosophila melanogaster / physiology
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Epistasis, Genetic
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Female
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Gene Expression Regulation, Developmental
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Infertility
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Male
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Meiosis / physiology*
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Mitosis / physiology*
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Molecular Sequence Data
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Oocytes / cytology
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Oocytes / physiology
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Ovary / cytology
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Ovary / physiology
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Phenotype
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Phosphorylation
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Protein Processing, Post-Translational
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Recombination, Genetic
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transgenes
Substances
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Actins
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Cell Cycle Proteins
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DNA-Binding Proteins
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Drosophila Proteins
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PLU protein, Drosophila
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Recombinant Fusion Proteins
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Transcription Factors
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gnu protein, Drosophila
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png protein, Drosophila
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Protein Serine-Threonine Kinases