Abstract
Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers. We also report on the presence of a membrane protein that may be responsible for bleomycin internalization and toxicity in Saccharomyces cerevisiae.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antibiotics, Antineoplastic / pharmacokinetics*
-
Bleomycin / pharmacokinetics*
-
Cell Membrane / metabolism
-
Cell Membrane Permeability / drug effects
-
Cell Membrane Permeability / physiology
-
Cell Wall / metabolism
-
Drug Resistance, Fungal
-
Electroporation
-
Fungal Proteins / metabolism
-
Membrane Proteins / metabolism
-
Saccharomyces cerevisiae / drug effects
-
Saccharomyces cerevisiae / metabolism*
-
Spheroplasts / physiology
Substances
-
Antibiotics, Antineoplastic
-
Fungal Proteins
-
Membrane Proteins
-
Bleomycin