Abstract
The modulation of synaptic plasticity by serotonin type II (5-hydroxytryptamine type II (5-HT(2)))-receptor stimulation was explored using intracellular, field potential and Fura-2 fluorescence image recordings in a rat amygdala slice preparation. Bath application of 5HT(2) receptor agonist 1-(2,5)-dimethoxy-4-iodophen-2-aminopropane (DOI) transformed theta-burst-stimulated (TBS) synaptic plasticity from short-term potentiation to long-term potentiation. DOI enhanced N-methyl-D-aspartate (NMDA) receptor-mediated potentials and calcium influx without affecting the resting membrane potential or input resistance of the neurons. In contrast, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/kainate receptor-mediated excitatory synaptic responses were unaffected by DOI. The facilitating effects of DOI were blocked by the 5-HT(2) receptor antagonist, ketanserin, and by the 5-HT(2C)-receptor selective antagonist, RS102221. These results indicate that 5-HT(2)-receptor activation enhances NMDA receptor-mediated synaptic function in the basolateral amygdala (BLA).
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amygdala / anatomy & histology
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Amygdala / drug effects*
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Amygdala / physiology
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Animals
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Calcium / metabolism
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Dose-Response Relationship, Drug
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Drug Synergism
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Electric Stimulation
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Excitatory Amino Acid Agonists / pharmacology*
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Fluorescent Dyes / metabolism
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Fura-2 / metabolism
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Glycine / pharmacology
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In Vitro Techniques
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Indophenol / analogs & derivatives*
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Indophenol / pharmacology*
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Ketanserin / pharmacology
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Magnesium / pharmacology
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Male
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Membrane Potentials / drug effects
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N-Methylaspartate / pharmacology*
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Neuronal Plasticity / drug effects*
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Rats
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Rats, Sprague-Dawley
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Serotonin Antagonists / pharmacology
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Serotonin Receptor Agonists / pharmacology*
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Spiro Compounds / pharmacology
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Sulfonamides / pharmacology
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Synaptic Transmission / drug effects
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Time Factors
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Valine / analogs & derivatives*
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Valine / pharmacology
Substances
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8-(5-(5-amino-2,4-dimethoxyphenyl)-5-oxopentyl)-1,3,8-triazaspiro(4.5)decane-2,4-dione
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Excitatory Amino Acid Agonists
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Fluorescent Dyes
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Serotonin Antagonists
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Serotonin Receptor Agonists
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Spiro Compounds
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Sulfonamides
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dimethoxy-4-indophenyl-2-aminopropane
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Indophenol
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N-Methylaspartate
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2-amino-5-phosphopentanoic acid
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Ketanserin
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Valine
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Magnesium
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Calcium
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Glycine
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Fura-2