Effect of cadmium and zinc ethanolamine complexes on rat brain monoamine oxidase-B activity in vitro

J Inorg Biochem. 2003 Jun 1;95(2-3):141-8. doi: 10.1016/s0162-0134(03)00096-5.

Abstract

Monoamine oxidase-B (MAO-B) from rat brain was inhibited strongly by the prepared cadmium and zinc ethanolamine complexes obtained from their sulphate and chloride salts. The inhibition of MAO-B by these complexes was time-dependent and fully reversible after dilution and sedimentation. In vitro, the cadmium ethanolamine complexes were more potent at inhibiting MAO-B than the zinc complexes. The inhibitory effect of these complexes follow the order: TEA>DEA>MEA, due to the alkyl residues and steric effect properties. The inhibition of MAO-B by cadmium and zinc ethanolamine complexes was a noncompetitive type. The K(i) values were calculated. The influence of the complexes on the activity of MAO-B was rather evaluated. It decreased the MAO-B activity. The IC(50) values of the two potent cadmium and zinc triethanolamine complexes on MAO-B were evaluated indicating that the complexes were tightly binding, but reversible inhibitors for MAO-B. In general, these systems may be used for preventing some neurodegenerative diseases.

MeSH terms

  • Animals
  • Benzylamines / metabolism
  • Brain / drug effects
  • Brain / enzymology*
  • Cadmium / chemistry
  • Cadmium / pharmacology*
  • Centrifugation / methods
  • Dialysis
  • Enzyme Stability
  • Ethanolamine / chemistry
  • Ethanolamine / pharmacology*
  • Inhibitory Concentration 50
  • Kinetics
  • Monoamine Oxidase / metabolism*
  • Monoamine Oxidase Inhibitors / pharmacology*
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / pharmacology
  • Rats
  • Stereoisomerism
  • Structure-Activity Relationship
  • Zinc / chemistry
  • Zinc / pharmacology*

Substances

  • Benzylamines
  • Monoamine Oxidase Inhibitors
  • Organometallic Compounds
  • Cadmium
  • Ethanolamine
  • benzylamine
  • Monoamine Oxidase
  • Zinc