Objective: To investigate the development and mechanism of the p16 gene inactivation in laryngeal squamous cell carcinoma (LSCC).
Methods: With PCR-based methylation assay and PCR-SSCP, first and second exon mutation in p16 gene and methylation of endogenase in promoter in 32 cases of LSCC and paracancer tissue were studied.
Results: No mutation were found in first and second exon in the samples; methylation of Cfo I were detected in 6 cases in first exon, methylation of Sac II was found in 4 cases, and methylation of Hpa II in 4 cases. In second exon, methylation of Hpa II was detected in 5 cases and methylation of Cfo I in 6 cases.
Conclusion: Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene. The inactivation of p16 gene has a close relation with the development and progress of LSCC.