Abstract
The domestic cat (Felis domesticus) is an important cause of allergic disease worldwide. The major cat allergen 1 (Fel d 1) has been expressed in Escherichia coli, purified and refolded in a soluble form. Crystals of Fel d 1 were obtained in 13% 2-methyl-2,4-pentanediol, 0.1 M sodium acetate pH 4.8. The Fel d 1 crystals belong to space group P2(1), with unit-cell parameters a = 43.3, b = 51.5, c = 67.7 A, and diffract to 1.9 A resolution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allergens / biosynthesis
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Allergens / chemistry*
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Allergens / isolation & purification
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Animals
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Cats
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Electrophoresis, Polyacrylamide Gel
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Glycoproteins / biosynthesis
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Glycoproteins / chemistry*
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Glycoproteins / isolation & purification
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / genetics
Substances
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Allergens
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Glycoproteins
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Recombinant Proteins
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Fel d 1 protein, Felis domesticus