Enantiomeric separation of acidic compounds of pharmaceutical interest by capillary electrochromatography employing glycopeptide antibiotic stationary phases

J Chromatogr A. 2003 Apr 25;994(1-2):227-32. doi: 10.1016/s0021-9673(03)00439-4.

Abstract

Enantiomeric separation of some selected acidic compounds of pharmaceutical interest belonging to the group of non-steroidal anti-inflammatory drugs were separated by capillary electrochromatography employing silica based glycopeptide antibiotic stationary phases, namely vancomycin or a teicoplanin derivatives (Hepta-Tyr). The vancomycin stationary phase allowed to achieve the chiral resolution of some racemic studied compounds only using mobile phases containing ammonium formate at a relatively low pH 2.5-3.5 and acetonitrile. Employing the teicoplanin derivative stationary phase, good enantiomeric resolution was achieved eluting with mobile phases containing sodium phosphate pH 6-acetonitrile. Enantiomers were moved to the detector because a relatively high reversed electroosmotic flow (due to the positive charge of the stationary phase) and to the electrophoretic mobility of analytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids
  • Anti-Bacterial Agents / chemistry*
  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Glycopeptides*
  • Pharmaceutical Preparations / chemistry*
  • Stereoisomerism

Substances

  • Acids
  • Anti-Bacterial Agents
  • Glycopeptides
  • Pharmaceutical Preparations