Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides

J Am Chem Soc. 2003 May 21;125(20):6160-4. doi: 10.1021/ja034342r.

Abstract

Carbon nanotubes (NTs) are becoming highly attractive molecules for applications in medicinal chemistry. The main problem of insolubility in aqueous media has been solved by developing a synthetic protocol that allows highly water-soluble carbon NTs to be obtained. As a result, biologically active peptides can be easily linked through a stable covalent bond to carbon NTs. We have demonstrated that a bound peptide from the foot-and-mouth disease virus, corresponding to the 141-159 region of the viral envelope protein VP1, retained the structural integrity and was recognized by monoclonal and polyclonal antibodies. In addition, this peptide-NT conjugate is immunogenic, eliciting antibody responses of the right specificity. Such a system could be greatly advantageous for diagnostic purposes and could find future applications in vaccine delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / chemistry*
  • Capsid Proteins / immunology*
  • Chromatography, High Pressure Liquid
  • Epitopes, B-Lymphocyte / chemistry
  • Epitopes, B-Lymphocyte / immunology
  • Foot-and-Mouth Disease Virus / immunology
  • Nanotechnology / methods
  • Nanotubes, Carbon / chemistry*
  • Nanotubes, Carbon / immunology*
  • Nuclear Magnetic Resonance, Biomolecular
  • Peptide Fragments / chemistry*
  • Peptide Fragments / immunology*

Substances

  • Capsid Proteins
  • Epitopes, B-Lymphocyte
  • Nanotubes, Carbon
  • Peptide Fragments
  • VP1 protein, Foot-and-mouth disease virus