Abstract
DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2',5'-Oligoadenylate Synthetase / metabolism*
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Enzyme Induction
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Fibroblasts / metabolism
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Genetic Vectors*
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HeLa Cells
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Humans
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Interferons / metabolism*
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Lentivirus / genetics*
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Lung / metabolism
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Promoter Regions, Genetic
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RNA / chemistry*
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RNA / metabolism*
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RNA Polymerase III / metabolism
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RNA, Small Interfering / metabolism*
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Transcription Factors / antagonists & inhibitors
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
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Transcription, Genetic
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Transfection
Substances
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MORF4 protein, human
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RNA, Small Interfering
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Transcription Factors
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RNA
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Interferons
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OAS1 protein, human
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RNA Polymerase III
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2',5'-Oligoadenylate Synthetase