Abstract
The cDNAs of two types of fatty acid-binding protein (FABP) present in human kidney, previously described as types A and B, were isolated using reverse transcriptase-PCR (RT-PCR) with human kidney mRNA and various sets of primers. The cDNA fragments were cloned and sequenced. Renal FABP type A and B cDNAs appeared to be completely identical to human liver- and heart-type FABP cDNAs respectively. In the second part of this study we demonstrated the presence of liver-type FABP in rat kidney by chromatography, e.l.i.s.a. and immunocytochemistry. The ratio and cellular distribution of the two FABP types varies markedly in human and rat kidney. Using RT-PCR we were also able to prepare and identify liver- and heart-type FABP cDNAs with mRNA from both male and female rat kidney.
MeSH terms
-
Animals
-
Base Sequence
-
Carrier Proteins / analysis
-
Carrier Proteins / genetics*
-
DNA / chemistry
-
DNA / genetics
-
DNA / isolation & purification
-
Fatty Acid-Binding Protein 7
-
Fatty Acid-Binding Proteins
-
Humans
-
Immunoenzyme Techniques
-
Liver / chemistry*
-
Molecular Sequence Data
-
Myocardium / chemistry*
-
Neoplasm Proteins*
-
Nerve Tissue Proteins*
-
Nucleic Acid Hybridization
-
Polymerase Chain Reaction
-
RNA, Messenger / analysis
-
RNA-Directed DNA Polymerase
-
Rats
-
Rats, Wistar
-
Sequence Homology, Nucleic Acid
-
Tissue Distribution
-
Transcription, Genetic
-
Tumor Suppressor Proteins*
Substances
-
Carrier Proteins
-
FABP7 protein, human
-
Fabp7 protein, rat
-
Fatty Acid-Binding Protein 7
-
Fatty Acid-Binding Proteins
-
Neoplasm Proteins
-
Nerve Tissue Proteins
-
RNA, Messenger
-
Tumor Suppressor Proteins
-
DNA
-
RNA-Directed DNA Polymerase