Abstract
In the absence of appropriate stimuli, polymorphonuclear neutrophils (PMN) undergo programmed cell death (PCD), also termed apoptosis. We show that granulocyte-macrophage colony-stimulating factor (GM-CSF), but not the chemotactic factors formyl-methionyl-leucyl-phenylalanine (FMLP), recombinant human (rh) C5a, transforming growth factor (TGF)-beta, and interleukin-8 (IL-8), or other cytokines including IL-3, IL-4, IL-6, and G-CSF, maintains viability of PMN in culture by preventing these cells from undergoing PCD. Prevention from PCD by GM-CSF was associated with induction of RNA and protein synthesis in PMN. Inhibition of RNA and protein synthesis by actinomycin-D and cycloheximide impeded the protection of apoptosis by GM-CSF. Similarly, neutralization of GM-CSF biologic activity by a specific antiserum abrogated GM-CSF-mediated inhibition of PCD.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apoptosis / drug effects*
-
Cell Survival / drug effects*
-
Complement C5a / pharmacology
-
Cycloheximide / pharmacology
-
Cytokines / pharmacology*
-
Dactinomycin / pharmacology
-
Female
-
Granulocyte Colony-Stimulating Factor / pharmacology
-
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
-
Humans
-
Interleukin-3 / pharmacology
-
Interleukin-4 / pharmacology
-
Interleukin-6 / pharmacology
-
Interleukin-8 / pharmacology
-
Kinetics
-
Male
-
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
-
Neutrophils / cytology*
-
Neutrophils / drug effects
-
Recombinant Proteins / pharmacology
-
Time Factors
-
Transforming Growth Factor beta / pharmacology
Substances
-
Cytokines
-
Interleukin-3
-
Interleukin-6
-
Interleukin-8
-
Recombinant Proteins
-
Transforming Growth Factor beta
-
Granulocyte Colony-Stimulating Factor
-
Dactinomycin
-
Interleukin-4
-
N-Formylmethionine Leucyl-Phenylalanine
-
Complement C5a
-
Granulocyte-Macrophage Colony-Stimulating Factor
-
Cycloheximide