Abstract
To investigate the effect of global cerebral ischemia on brain cell proliferation in young adult macaques, we infused 5-bromo-2'-deoxyuridine (BrdU), a DNA replication indicator, into monkeys subjected to ischemia or sham-operated. Subsequent quantification by BrdU immunohistochemistry revealed a significant postischemic increase in the number of BrdU-labeled cells in the hippocampal dentate gyrus, subventricular zone of the temporal horn of the lateral ventricle, and temporal neocortex. In all animals, 20-40% of the newly generated cells in the dentate gyrus and subventricular zone expressed the neural progenitor cell markers Musashi1 or Nestin. A few BrdU-positive cells in postischemic monkeys were double-stained for markers of neuronal progenitors (class III beta-tubulin, TUC4, doublecortin, or Hu), neurons (NeuN), or glia (S100beta or GFAP). Our results suggest that ischemia activates endogenous neuronal and glial precursors residing in diverse locations of the adult primate central nervous system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biomarkers
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Brain Ischemia / metabolism*
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Brain Ischemia / physiopathology
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Bromodeoxyuridine
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Cell Death / physiology
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Cell Division / physiology
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DNA Damage / physiology
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Dentate Gyrus / cytology
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Dentate Gyrus / growth & development
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Dentate Gyrus / metabolism
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Female
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Immunohistochemistry
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Intermediate Filament Proteins / metabolism
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Lateral Ventricles / cytology
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Lateral Ventricles / growth & development
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Lateral Ventricles / metabolism
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Macaca
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Nerve Regeneration / physiology*
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Nerve Tissue Proteins / metabolism
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Nestin
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Neuronal Plasticity / physiology*
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Neurons / cytology
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Neurons / metabolism*
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Phenotype
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RNA-Binding Proteins / metabolism
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Stem Cells / cytology
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Stem Cells / metabolism*
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Telencephalon / cytology
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Telencephalon / growth & development*
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Telencephalon / metabolism
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Temporal Lobe / cytology
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Temporal Lobe / growth & development
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Temporal Lobe / metabolism
Substances
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Biomarkers
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Intermediate Filament Proteins
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Nerve Tissue Proteins
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Nestin
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RNA-Binding Proteins
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Bromodeoxyuridine