Differential effects of the protein kinase C inhibitors H7 and calphostin C on the cell cycle of neuroblastoma cells

Brain Res. 1992 Nov 20;596(1-2):157-62. doi: 10.1016/0006-8993(92)91543-n.

Abstract

We have studied the effect of protein kinase C inhibitors 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7) and calphostin C on the cycle of Neuro-2a cells. Both compounds inhibited cell proliferation and DNA synthesis. Transition from G2 to M phase was not altered by these compounds. Calphostin C blocked the cells in G0/G1, while H7 did not at any specific point in the cell cycle. We also show that the antiproliferative effect induced by both inhibitors is reversible.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Animals
  • Cell Cycle / drug effects
  • Cell Membrane Permeability
  • Cell Survival / drug effects
  • DNA / antagonists & inhibitors
  • DNA / biosynthesis
  • Isoquinolines / pharmacology*
  • Light
  • Naphthalenes*
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology*
  • Piperazines / pharmacology*
  • Polycyclic Compounds / pharmacology*
  • Protein Kinase C / antagonists & inhibitors*
  • Sulfonamides*
  • Tumor Cells, Cultured

Substances

  • Isoquinolines
  • Naphthalenes
  • Piperazines
  • Polycyclic Compounds
  • Sulfonamides
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • DNA
  • N-(2-guanidinoethyl)-5-isoquinolinesulfonamide
  • Protein Kinase C
  • calphostin C