Antigen processing of the heptavalent pneumococcal conjugate vaccine carrier protein CRM(197) differs depending on the serotype of the attached polysaccharide

Infect Immun. 2003 Jul;71(7):4186-9. doi: 10.1128/IAI.71.7.4186-4189.2003.

Abstract

The pneumococcal (Pn) conjugate vaccine includes seven different polysaccharides (PS) conjugated to CRM(197). Utilizing antigen-processing cells and a CRM(197)-specific mouse T-cell hybridoma, we found that the serotype of conjugated PnPS dramatically affected antigen processing of CRM(197). Unconjugated CRM(197) and serotype conjugates 14 and 18C were processed more efficiently.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigen Presentation*
  • Bacterial Proteins / administration & dosage
  • Bacterial Proteins / metabolism*
  • HLA-DR1 Antigen / physiology
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Pneumococcal Vaccines / administration & dosage*
  • Polysaccharides, Bacterial / administration & dosage*
  • Serotyping
  • T-Lymphocytes / immunology
  • Vaccines, Conjugate / administration & dosage*

Substances

  • Bacterial Proteins
  • HLA-DR1 Antigen
  • Pneumococcal Vaccines
  • Polysaccharides, Bacterial
  • Vaccines, Conjugate
  • CRM197 (non-toxic variant of diphtheria toxin)