Immunohistochemical evaluation of PCNA, p53, HSP60, HSP10 and MUC-2 presence and expression in prostate carcinogenesis

Anticancer Res. 2003 Mar-Apr;23(2B):1325-31.

Abstract

Background: The study of the expression of different biological markers in non-neoplastic, pre-neoplastic and neoplastic lesions of prostate could help to better understand their role in carcinogenesis and to find new diagnostic and prognostic tools.

Materials and methods: In the present work we evaluated, by immunohistochemistry, the presence and the expression of PCNA, p53, HSP60, HSP10 and MUC-2 in a series of nodular hyperplasia, low- and high-grade prostatic intraepithelial lesions and adenocarcinomas.

Results: Our data confirmed that: 1) PCNA expression could be related to the grade of progression of cancer; and that 2) p53 mutation could be a late event in prostate carcinogenesis. Moreover, we reported that: 1) HPS60 and HPS10 were overexpressed early in prostate carcinogenesis; and that 2) MUC-2 is absent in both tumoral and non-tumoral prostatic tissue.

Conclusion: We suggest the further examination, by molecular and genetic studies, of the role of HSP60 and HSP10 during carcinogenesis of the prostate as well as of other organs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / chemistry*
  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism
  • Cell Differentiation
  • Cell Transformation, Neoplastic / genetics
  • Chaperonin 10 / analysis*
  • Chaperonin 10 / biosynthesis
  • Chaperonin 10 / genetics
  • Chaperonin 60 / analysis*
  • Chaperonin 60 / biosynthesis
  • Chaperonin 60 / genetics
  • Disease Progression
  • Gene Expression Profiling
  • Genes, p53
  • Humans
  • Immunohistochemistry
  • Male
  • Mucin-2
  • Mucins / analysis*
  • Mucins / biosynthesis
  • Mucins / genetics
  • Neoplasm Proteins / analysis*
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Proliferating Cell Nuclear Antigen / analysis*
  • Proliferating Cell Nuclear Antigen / biosynthesis
  • Proliferating Cell Nuclear Antigen / genetics
  • Prostate / metabolism*
  • Prostatic Hyperplasia / metabolism*
  • Prostatic Intraepithelial Neoplasia / chemistry*
  • Prostatic Intraepithelial Neoplasia / genetics
  • Prostatic Intraepithelial Neoplasia / metabolism
  • Prostatic Neoplasms / chemistry*
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / metabolism
  • Tumor Suppressor Protein p53 / analysis*
  • Tumor Suppressor Protein p53 / biosynthesis

Substances

  • Chaperonin 10
  • Chaperonin 60
  • MUC2 protein, human
  • Mucin-2
  • Mucins
  • Neoplasm Proteins
  • Proliferating Cell Nuclear Antigen
  • Tumor Suppressor Protein p53