Objective: To explore the safety of 5 mm cutting-edges with the analysis of DNA contend and cell cycle and the pathology observation.
Method: Twenty specimens of laryngocarcinoma and its cutting-edges were analyzed by flow cytometry, contrasting to 5 samples of normal larynx tissue, and their light-microscopy and electra-microscopy were discussed.
Result: The DNA aneuploidy(AN) rate in the laryngocarcinoma, its cutting-edges and the normal tissue is respectively 60% (12/20), 15%(3/20) and 0. The rate of S phrase of the laryngocarcinoma and its cutting-edges is respectively 20.33 +/- 6.0 and 12.35 +/- 3.81, so the DI 1.78 +/- 0.82 and 1.07 +/- 0.04, PI 29.09 +/- 6.8 and 11.75 +/- 3.75, there are significant differences (P < 0.05). The light-microscopy showed 5 mm cutting-edges were infiltrated by carcinoma in the form of submucosa or extention of carcinoma in situ and the results was, to some extension, consistent with the findings of FCM.
Conclusion: The safety of 5 mm cutting-edge varies depending on whether invading paraglottic space.