Simple and rapid technique for monitoring the quality of meningococcal polysaccharides by high performance size-exclusion chromatography

J Biochem Biophys Methods. 2003 Jun 30;56(1-3):291-6. doi: 10.1016/s0165-022x(03)00066-6.

Abstract

The molecular size of meningococcal polysaccharides is an important physico-chemical parameter which correlates with immunogenicity. This paper describes the experimental conditions for high-performance size-exclusion chromatography on a PL Aquagel-OH 60 column to follow changes in the size distribution and therefore in the distribution coefficient (K(D)) of the meningococcal polysaccharides of groups A, C, Y and W-135 used to formulate anti-Neisseria meningitidis vaccines. The experimental conditions were also found to be suitable for a rapid monitoring of the quality (no group A polysaccharide depolymerization) of the tetravalent meningococcal polysaccharide vaccine.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / analysis*
  • Antigens, Bacterial / chemistry*
  • Chromatography, Gel / methods*
  • Drug Stability
  • Gels / chemistry
  • Meningococcal Vaccines / analysis*
  • Meningococcal Vaccines / chemistry*
  • Molecular Weight
  • Polysaccharides, Bacterial / analysis*
  • Polysaccharides, Bacterial / chemistry*
  • Quality Control

Substances

  • Antigens, Bacterial
  • Gels
  • Meningococcal Vaccines
  • Polysaccharides, Bacterial