The production of hybridoma cell line secreting monoclonal antibodies against Xanthomonas campestris pv. oryzae and its application in the classification of strains

Chin J Biotechnol. 1992;8(2):139-44.

Abstract

By the fusion of mouse myeloma cells (SP2/0-Ag14) and spleen cells derived from BALB/c mice immunized with the preparation of Xanthomonas campestris pv. oryzae Ks-6-6, Os-213, Yz-32 and Yz-24, we obtained 12 hybridoma cell lines secreting monoclonal antibodies. None of the McAbs cross-reacted with the other varieties of plant pathogenetic and non-pathogenetic bacteria. The McAbs could distinguish three variant serotypes of strains. Antibody titers of ascites were about 1:10(3)-1:10(6) when measured by ELISA method. The McAbs could differentiate 6 epitopes. Based on the epitopes, the 63 strains of X. campestris pv. oryzae we collected were grouped into nine groups.

MeSH terms

  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Antibodies, Monoclonal / biosynthesis*
  • Antigens, Bacterial
  • Bacterial Typing Techniques
  • Biotechnology
  • Cross Reactions
  • Epitopes
  • Hybridomas / immunology*
  • Mice
  • Xanthomonas campestris / classification*
  • Xanthomonas campestris / immunology*
  • Xanthomonas campestris / pathogenicity

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Epitopes