Cutting edge: protective cell-mediated immunity to Listeria monocytogenes in the absence of myeloid differentiation factor 88

J Immunol. 2003 Jul 15;171(2):533-7. doi: 10.4049/jimmunol.171.2.533.

Abstract

In addition to their role in triggering innate immune responses, Toll-like receptors are proposed to play a key role in linking the innate and adaptive arms of the immune response. The majority of cellular responses downstream of Toll-like receptors are mediated through the adapter molecule myeloid differentiation factor 88 (MyD88), and mice with a targeted deletion of MyD88 are highly susceptible to bacterial infections, including primary infection with Listeria monocytogenes (LM). In contrast, herein we demonstrate that MyD88-deficient mice have only a modest impairment in their LM-specific CD4 T cell response, and no impairment in their CD8 T cell response following infection with ActA-deficient LM. Furthermore, CD8 T cells from immunized MyD88-deficient mice protected naive recipient mice following adoptive splenocyte transfer, and immunized MyD88-deficient mice were protected from infection with wild-type LM. These results indicate that adaptive immune responses can be generated and provide protective immunity in the absence of MyD88.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adoptive Transfer
  • Animals
  • Antigens, Differentiation / genetics*
  • Antigens, Differentiation / physiology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / microbiology
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / microbiology
  • Dose-Response Relationship, Immunologic
  • Epitopes, T-Lymphocyte / immunology
  • Genetic Predisposition to Disease
  • Immunity, Cellular / genetics
  • Immunity, Innate / genetics
  • Listeria monocytogenes / genetics
  • Listeria monocytogenes / immunology*
  • Listeriosis / genetics*
  • Listeriosis / immunology*
  • Listeriosis / microbiology
  • Lymphocyte Activation / genetics
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myeloid Differentiation Factor 88
  • Receptors, Immunologic / deficiency*
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / physiology
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / transplantation

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, Differentiation
  • Epitopes, T-Lymphocyte
  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • Receptors, Immunologic