Voltammetrically determined differences in changes evoked by KCl microinjections on catecholamine levels in the reticular formation and corpus striatum of the rat

Physiol Res. 1992;41(3):191-9.

Abstract

Using a microelectrode with carbon filaments and the voltammetric technique, changes evoked in the catechol oxidation current (CA.OC) and multiple unit activity (MUA) by microinjection of 3-5 microliters 0.5 mol.l-1 KCl were studied in the reticular formation (RF) of the medulla oblongata of anaesthetized rats; the effect of KCl stimulation of the RF and corpus striatum (S) on the CA.OC in these structures was compared. The microinjection of KCl in the vicinity of the working electrode in the RF caused depression of MUA which began 2-3 s after administration, persisted for up to 6 min after and then diminished, reaching control values within 9 min. The voltammetric signal was first recorded in the 1st min after microinjection, when there was an evident decrease in the CA.OC value (59% of the control value); this effect reached its maximum 7 min after administration (a mean drop to 23% of the control), while at the end of the experiment (i.e. after 24 min) CA.OC values had risen to 45-80% of the control value. The response in the S had a biphasic character, however. Immediately after the microinjection (1st min), the mean CA.OC value rose to 626% of the control, while in the second phase (3-10 min) it was seen to fall below the control values (means 21-63% of the control). The differences in the changes evoked by K+ depolarization in the concentration of catecholamines in the RF and S microenvironment are discussed from the aspect of the existence of different pools of the transmitter and other regional differences.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Catecholamines / biosynthesis*
  • Cell Membrane / drug effects
  • Cell Membrane / physiology
  • Corpus Striatum / metabolism*
  • Evoked Potentials
  • Extracellular Space / chemistry
  • Microinjections
  • Neurons / drug effects
  • Potassium Chloride / pharmacology*
  • Rats
  • Rats, Wistar
  • Reticular Formation / metabolism*
  • Time Factors

Substances

  • Catecholamines
  • Potassium Chloride