Faster genetic identification of medically important aspergilli by using gellan gum as gelling agent in mycological media

J Med Microbiol. 2003 Aug;52(Pt 8):653-655. doi: 10.1099/jmm.0.05135-0.

Abstract

Using gellan gum as a substitute for agar-agar in a mycological medium and sequencing of the ITS 1 and 2 regions resulted in an accurate identification of Aspergillus fumigatus, Aspergillus nidulans, Aspergillus terreus and Aspergillus ustus within 24 h of subculture.

MeSH terms

  • Aspergillus / classification*
  • Aspergillus / genetics*
  • Consensus Sequence
  • Culture Media / chemistry*
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / genetics
  • Mycological Typing Techniques / methods*
  • Polymerase Chain Reaction
  • Polysaccharides, Bacterial*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Time Factors

Substances

  • Culture Media
  • DNA, Fungal
  • DNA, Ribosomal Spacer
  • Polysaccharides, Bacterial
  • gellan gum