Abstract
In L2 mice, a high expression level of the transgenic lambda2(315) L chain results in nearly complete exclusion of endogenous L chains and a predominance of B-1a cells. In this study, we show that splenic and peritoneal B-1a cells differ considerably in their Ab repertoire and gene expression profile. Splenic B-1a cells exhibit a more diversified repertoire under L chain limitation. Despite oligoclonal overlaps between both B-1a compartments, some B cell receptor specificities are clearly restricted to the peritoneum. The capacity of peritoneal B-1a cells to enter the splenic B-1a compartment was found to be very limited. Gene expression profiling revealed genes up-regulated in splenic B-1a cells that are involved in mediating specialized first-line-of-defense effector functions and interaction with T cells. Thus, splenic and peritoneal B-1a cells differ not only in their developmental program but also in functional properties.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibody Diversity / genetics
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Antibody Specificity / genetics
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Antigens, CD / biosynthesis
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Ascitic Fluid / cytology
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Ascitic Fluid / immunology*
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Ascitic Fluid / metabolism
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B-Lymphocyte Subsets / cytology
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B-Lymphocyte Subsets / immunology*
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B-Lymphocyte Subsets / metabolism
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B7-1 Antigen / biosynthesis
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B7-2 Antigen
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Base Sequence
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell Movement / genetics
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Cell Movement / immunology
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Clone Cells
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Epitopes, B-Lymphocyte / immunology
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Gene Expression Profiling* / methods
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Immunoglobulin Heavy Chains / biosynthesis*
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Immunoglobulin Heavy Chains / blood
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Immunoglobulin Heavy Chains / genetics*
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Immunoglobulin Variable Region / biosynthesis*
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Immunoglobulin Variable Region / blood
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Immunoglobulin Variable Region / genetics*
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Membrane Glycoproteins / biosynthesis
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Mice
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Mice, Inbred BALB C
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Mice, Knockout
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Mice, Transgenic
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Molecular Sequence Data
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Multigene Family / immunology
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Phosphatidylcholines / immunology
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Species Specificity
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Spleen / cytology
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Spleen / immunology*
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Spleen / metabolism
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Up-Regulation / genetics
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Up-Regulation / immunology
Substances
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Antigens, CD
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B7-1 Antigen
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B7-2 Antigen
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Cd86 protein, mouse
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Epitopes, B-Lymphocyte
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Membrane Glycoproteins
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Phosphatidylcholines