Abstract
A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75:12359-12369, 2001), in which the ectodomain region of the spike gene from IBV M41-CK replaced the corresponding region of the IBV Beaudette genome. BeauR-M41(S) acquired the same cell tropism phenotype as IBV M41-CK in four different cell types, demonstrating that the IBV spike glycoprotein is a determinant of cell tropism.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA, Complementary
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Infectious bronchitis virus / genetics
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Infectious bronchitis virus / physiology*
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Molecular Sequence Data
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Recombination, Genetic*
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Spike Glycoprotein, Coronavirus
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Tropism*
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / physiology*
Substances
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DNA, Complementary
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Membrane Glycoproteins
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Spike Glycoprotein, Coronavirus
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Viral Envelope Proteins