In vitro and in vivo expression of somatostatin receptors in intermediate and malignant soft tissue tumors

Anticancer Res. 2003 May-Jun;23(3B):2465-71.

Abstract

Background: Previous studies showed that the subtype 2 of somatostatin receptors (sstr2) is generally expressed by soft tissue sarcomas.

Materials and methods: Using reverse transcription-polymerase chain reaction (RT-PCR), the present study evaluated the expression of sstr mRNA in 31 human intermediate and malignant soft tissue tumors. In 8 patients somatostatin receptor scintigraphy was performed to detect soft tissue tumor sites and visualize in vivo sstr expression.

Results: Somatostatin receptors were identified in 84% of the tumors analyzed by RT-PCR analysis. sstr1 and sstr2 were the receptors most frequently detected. Somatostatin receptor scintigraphy was able to identify primary and/or metastatic sites in 7 out of 8 patients, with an overall sensitivity per site of 87%. Concordant results were found between in vitro and in vivo techniques.

Conclusion: The wide expression of sstrs in soft tissue tumors suggest a relevant role for these receptors in diagnosis and may have therapeutic applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Fibroma / genetics
  • Fibroma / metabolism
  • Histiocytoma, Benign Fibrous / genetics
  • Histiocytoma, Benign Fibrous / metabolism
  • Humans
  • Leiomyosarcoma / genetics
  • Leiomyosarcoma / metabolism
  • Liposarcoma / genetics
  • Liposarcoma / metabolism
  • Male
  • Middle Aged
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptors, Somatostatin / biosynthesis*
  • Receptors, Somatostatin / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma / genetics
  • Sarcoma / metabolism*
  • Soft Tissue Neoplasms / genetics
  • Soft Tissue Neoplasms / metabolism*

Substances

  • RNA, Messenger
  • Receptors, Somatostatin