Objective: To explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells (BMSCs).
Methods: The marker gene, pbLacZ, was transferred into cultured BMSCs and the expression of transduced gene by X-gal staining was examined. Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected.
Results: The exogenous gene could be expressed efficiently in transduced BMSCs.
Conclusion: The present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.