Abstract
In vitro selection has been used as a method to determine the optimal binding site for DNA-binding proteins. We report here in vitro selection of dsDNA sequences that bind to mutated-GCN4-bZIP peptides. The GCN4-bZIP peptide mutated from alanine to histidine on a position-14 that contacts with DNA bound to different sequence from a binding site of wild type peptide.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Basic-Leucine Zipper Transcription Factors
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Binding Sites / genetics
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DNA / chemistry
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DNA / genetics
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DNA / metabolism*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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G-Box Binding Factors
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In Vitro Techniques
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Kinetics
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Mutagenesis
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Protein Binding
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Protein Kinases / chemistry
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Protein Kinases / genetics*
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Protein Kinases / metabolism*
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Quartz
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / metabolism*
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Selection, Genetic
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism*
Substances
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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G-Box Binding Factors
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Quartz
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DNA
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Protein Kinases